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Manufacturing Process of Prime Peptides Research-Grade Peptides

At Prime Peptides, we are dedicated to producing high-quality research-grade peptides with exceptional purity and consistency. We ensuring stringent quality control at every stage.

1. High-Purity Water Usage

The process begins with the dissolution of peptides in WFI (Water for Injection) grade water, the highest purity standard available. This ensures a clean and contamination-free foundation for our products.

2. Excipients for Stability

To enhance stability, mannitol and sucrose are added as excipients. Mannitol serves as a bulking agent, while sucrose acts as a lyoprotectant, preventing degradation and aggregation throughout the freeze-drying and storage process.

3. Electrospray Ionization (ESI) and Sodium Introduction

During the Electrospray Ionization (ESI) process, the peptide solution is sprayed into a fine mist. At this stage, sodium ions are introduced and readily attach to the peptide molecules, facilitating ionization. This controlled ionization process plays a crucial role in enhancing the stability and characterization of the peptides.

4. Sterile Filtration

Following ionization, the peptide solution undergoes sterile filtration through a 0.22 µm PES (polyethersulfone) filter membrane to remove any potential contaminants and ensure a high level of purity.

5. Precise Vialing and Freeze-Drying

Once filtered, the solution is precisely dispensed into 3 mL vials. The vials are partially stoppered and placed into a freeze-dryer for a carefully controlled 36-hour lyophilization cycle, which consists of:

• Freezing Phase: The solution is cooled to -40°C, stabilizing the peptide structure.
• Primary Drying: Water is removed through sublimation, eliminating the majority of moisture.
• Secondary Drying: Any remaining bound water is removed via desorption, ensuring a stable, lyophilized peptide.

This entire process, from preparation to freeze-drying, is conducted in our manufacturing facility, ensuring strict quality control, purity, and consistency in every batch.

At Prime Peptides, we are committed to delivering research-grade peptides that meet the highest standards of quality and reliability.